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Congresso Brasileiro de Microbiologia 2023
 		Resumo: 1284-2

1284-2

COVID-19 triggers exposure of endangered wildlife to hospital-associated WHO critical priority pathogens co-producing NDM and KPC carbapenemases

Autores:
Fabio Parra Sellera (USP - UNIVERSIDADE DE SAO PAULO) ; Brenda Cardoso (USP - UNIVERSIDADE DE SAO PAULO) ; Danny Fuentes-castillo (UC - Universidad de Concepción) ; Herrison Yoshiki Yocida Fontana (USP - UNIVERSIDADE DE SAO PAULO) ; Bruna Fuga (USP - UNIVERSIDADE DE SAO PAULO) ; Fernanda Esposito (USP - UNIVERSIDADE DE SAO PAULO) ; Nilton Lincopan (USP - UNIVERSIDADE DE SAO PAULO)

Resumo:
An epidemiological alert on the emergence and increase of carbapenem-resistant bacteria co-producing KPC and NDM carbapenemases in Latin America and the Caribbean — possiblyrelated to the increased use of broad-spectrum antibiotics in patients with COVID-19 — was announced by the Pan American Health Organization/World Health Organization (PHAO/WHO), emphasizing the importance of appropriate microbiological diagnosis and the effective and articulated implementation of infection prevention and control programs. In this regard, the COVID-19 pandemic has led to a steep and robust increase in antibiotic and biocide use worldwide, possibly contributing to increased concentrations of antimicrobials in human-waste receiving natural environments and consequent further spread of antibiotic-resistant bacteria and their resistance genes beyond hospital settings. Worryingly, during the COVID-19 pandemic an increased number of injured and diseased wild animals infected or colonized by multidrug-resistant bacteria, categorized as critical priority pathogens by WHO were admitted to rehabilitation centres in Brazil. As part of the Grand Challenges Explorations-New Approaches to Characterize the Global Burden of Antimicrobial Resistance program, we have performed a genomic investigation. Genomic DNA was obtained using PureLink Extraction Kit and sequencing performed on an Illumina NextSeq550 platform. De novo assembly was performed using Unicycler and in silico analysis performed using bioinformatic tools. We identified 18 clinically relevant pathogens classified as critical priority by the World Health Organization (WHO), including NDM-1, KPC-2, OXA-23 and IMP-16-producing Escherichia coli, Enterobacter cloacae, Citrobacter freundii, Pseudomonas putida, Acinetobacter baumannii, and hospital-associated clones of Klebsiella quasipneumoniae sequence type ST1308 co-producing NDM-1 and KPC-2, in seabirds, green turtles, whale, and penguins with fatal outcomes, alerting for an unprecedented event of epidemiological and ecological significance worldwide. Since Brazil hosts between 15-20% of the world's biological diversity, with the greatest number of endemic species on a global scale, our findings are remarkably important because may point out that vulnerable wildlife is been threatened by carbapenemase-producing pathogens under the shadow of the COVID-19 crisis.

Palavras-chave:
 wildlife, carbapenemases, genomic investigation


Agência de fomento:
Bill & Melinda Gates Foundation (Grand Challenges Explorations Brazil OPP1193112)